Biosorption of lead from aqueous solution by fungal biomass of Aspergillus niger and Rhizopus sp.

  • มธุรส อ่อนไทยและวราภรณ์ พันธุ์ศุภผล
Keywords: Fungal biomass, Biosorption, Lead, Aspergillus niger, Rhizopus sp.


The biosorption of lead from aqueous solution by pretreated fungal biomass of Aspergillus niger and Rhizopus sp. with NaOH was investigated. Parameters affecting biosorption such as pH, temperature and contact time were examined. It was found that the initial pH of the solution strongly affected to the degree of biosorption. The pH of 5 and 6 were the optimum pH on biosorption of Rhizopus sp. and A. niger, respectively. The wide range of temperature of 30-60°C showed slightly effect on lead adsorption. The rapid rate of adsorption was found in during the first 60 min and was remained nearly constant afterwards. The optimum contact time was between 60 and 90 min. Moreover, the lead adsorption by Rhizopus sp. was higher than that of A. niger in all cases of the study conditions.


1. Feng Q, Lin Q, Gong F, Sugita S, Shoya M. Adsorption of lead and mercury by rice huskash. J Colloid Interface Sci 2004;278(1):1-8.
2. Selatnia A, Boukazoula A, Kechid N, Bakhti MZ, Chergui A, Kerchich Y. Biosorption of lead (II) from aqueous solution by a bacterial dead Streptomyces rimosus biomass. Biochem Eng J 2004;19(2):127-35.
3. Kapoor A, Viraraghavan T. Fungal biosorption -an alternative treatment option for heavy metal bearing wastewaters: a review. Bioresource Technol 1995;53(3):195-206.
4. Kapoor A, Viraraghavan T, Cullimore DR. Removal of heavy metals using the fungus Aspergillus niger. Bioresource Technol 1999;
5. Das N, Vimala R, Karthika P. Biosorption of heavy metals - an overview. Indian J Biotechnol 2008;7(2):159-69.
6. Hafez N, Abdel-Razek AS, Hafez MB. Accumulation of some heavy metals on Aspergillus flavus. J Chem Tech Biotechnol 1997;68(1):19-22.
7. Veglio F, Beolchini F. Removal of metals by biosorption: a review. Hydrometallurgy 1997;44(3):301-16.
8. Kapoor A, Viraraghavan T. Biosorption of heavy metals on Aspergillus niger: Effect of pretreatment. Bioresour Technol 1998;63(2):109-13.
9. Bahadir T, Bakan G, Altas L, Buyukgungor H. The investigation of lead removal by biosorption: an application at storage battery industry wastewaters. Enzyme Microb Tech 2007;41 (1-2):98-102.
10. Dhankhar R, Hooda A. Fungal biosorption - an alternative to meet the challenges of heavy metal pollution in aqueous solutions. Environ Technol 2011;32(5):467-91.
11. Jianlong W, Xinmin Z, Decai D, Ding Z. Bioadsorption of lead(II) from aqueous solution by fungal biomass of Aspergillus niger. J Biotechnol 2001;87(3):273-7.
12. Naja G, Mustin C, Berthelin J, Volesky B. Lead biosorption study with Rhizopus arrhizus using a metal - based titration technique. J Colloid Interf Sci 2005;292(2):537-43.
13. Amini M, Younesi H, Bahramifar N, Lorestani AAZ, Ghorbani F, Daneshi A, et al. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. J Hazard Mater 2008;154(1-3):694-702.
14. Gomes PF, Lennartsson PR, Persson NK, Taherzadeh MJ. Heavy metal biosorption by Rhizopus sp. biomass immobilized on textiles. Water Air Soil Poll 2014;225(2):1-10.
15. Akar T, Tunali S, Kiran I. Botrytis cinerea as a new fungal biosorbent for removal of Pb(II) from aqueous solutions. Biochem Eng J 2005;25(3):227-35.
16. Muzzarelli RAA, Tanfani F, Scarpini G, Muzzarelli MG. ESR characterization of chitins and chitosans. Biochem Bioph Res Co 1979;89(2):706-12.
17. Muzzarelli RAA, Tanfani F, Scarpini G, Tucci E. Removal and recovery of cupric and mercuric ions from solutions using chitosan-glucan from Aspergillus niger. J Appl Biochem 1980;2(1):54-9.
18. Dursun AY. A comparative study ondetermination of the equilibrium, kinetic and thermodynamic parameters of biosorption of copper(II) and lead(II) ions onto pretreated Aspergillus niger. Biochem Eng J 2006;28(2):187- 95.
19. Akar T, Tunali S. Biosorption characteristics of Aspergillus flavus biomass for removal of Pb(II)and Cu(II) ions from an aqueous solution. Bioresource Technol 2006;97(15):1780-7